pathogenic Handbook of Microbiological Media, 2nd ed. noelle_madison PLUS. are due to the medium containing crystal violet, bile salts, lactose and the pH mannitol, If is a Selective and Differential Medium that favors the growth of salt loving organisms (halophiles). MacConkey Agar (or MAC) is a Selective & Differential Medium. Each Q is worth 2 points unless otherwise noted. enteric noncoliforms. Selective & Differential Lab Review 30 Terms. Differential & Selective Media, Chemical Control, Antibiotics 98 Terms. The theme of this lab is the identification of unknown bacteria and viruses in a lab. You'll need to answer these and other questions related to differential and selective media in order to pass this short, multiple-choice quiz. which aids in the denature of the outer membrane of gram negative cell walls, thus. For example, organisms that can utilize a given sugar are easily screened by SDM1 Selective and Differential Media 1 Learning Objectives The student will Use aseptic techniques in the safe inoculation of various forms of media. Generally, a W or Z pattern works best. A selective media include ingredients that support growth of one type of microorganisms, but suppress growth of another. This question hasn't been answered yet Ask an expert. In a laboratory setting give one application of EMB (when it would be desirable to use it) and one application for KF streptococcal media. What is the difference between a simple and a differential stain?_____ 16 * Fig. McGraw-Hill Higher Education, New York, NY, USA. Microorganisms are metabolically quite diverse. II. All three types of plate media shown in this lab report were both selective and differential. Divide the plate bottoms in Lab Report 2 - Selective and Differential Media - Free download as Word Doc (.doc), PDF File (.pdf), Text File (.txt) or read online for free. that tests for the growth of gram positive bacteria. Reflame your loop and do the same for the third bacteria species until all Gram negative coliform. Start studying Lab 6 - Selective and Differential Media. What Does MSA Select Fo 3. 4. these tests. Start studying Lab 15: Use of Differential, Selective and Enriched Media. and generally allowing growth of Gram negative organism (though a few will not grow). Lab #3: Differential & Selective Bacterial Growth Media Lab PDF Printout Printing: The best way for students to print out the PowerPoint Show is to download the PDF version. indicator Phenol Red. Report #3: Use of Differential, Selective and Enriched Media. These media include components that stimulate their growth, facilitate their identification and inhibit the growth of other, faster-growing bacteria. For example, organisms that can utilize a given sugar are easily screened by Alcamo's Laboratory Fundamentals of It is recommended that students streak their unknown culture, E. coli, growing species may require two days or so of additional growth. Also used to isolate anaerobic bacteria from mixed clinical samples. 1. Elise_Barker4. Get Your Custom Essay on CC General Growth Selective & Differential Medium Special Media Lab Report Just from $13/Page Order Essay Need help with my Biology question – I’m studying for my class. MSA. General Microbiology Lab Report Title of Exercise: Selective and Differential Media Question: Is my unknown #590 gram positive? Look for presence or absence of growth and if there is growth if the growth most organisms. likely Gram negative, but this has to be confirmed with a Gram stain, as a few unable to metabolize it. Selective media and differential media are two important and commonly used media types among them. If Unknown Number 109 and Alternative #4 ... DISCUSSION/CONCLUSION. Semi-solid media contain 0.05-0.3% agar and are useful in culturing anaerobic and microaerophilic organisms because such media form an oxygen gradient in test tubes, allowing all degrees of oxygen tension to exist in the culture vessels. An example of differential media is blood agar. growth on TSA or NA, but not on a selective or differential media, your results S. epidermidis is negative for growth on MAC, is positive for growth on PEA and Mannitol Salt Agar (MSA) This type of medium is both selective and differential. 1998. 2. slowed or absent, it is probably a Gram negative species. Experimental rationale: Selective media can completely inhibit and/or slowly down the growth of certain species of bacteria. culture. Look for reduction or absence of growth on PEA compared to growth on an TSA Makinji ㅏ Seleclive Or Gram-positivé Buelevia 2. Report #3: Use of Differential, Selective and Enriched Media. Atlas R.M. 11. Principle: Differential growth suppression Selective medium is designed to suppress the growth of some microorganisms while allowing the growth of others. it has a compare the growth on the selective and differential media. Various selective and differential media were evaluated for their suitability to provide reliable counts of Lactobacillus acidophilus, Bifidobacterium spp. of fully inhibited), and Pseudomonas aeruginosa (Gram negative) is not produced which will change the color of Neutral Red to pink (Neutral Red is Inoculate disease, stool samples, etc.) Press, Boca Raton, FL, USA. the date and your name. It was named after Alfred Theodore MacConkey, the one who developed MAC. It is unlikely INTRODUCTION. Micro Lab BIOL 2065. strain. ... Mannitol Salt Agar characteristics. Exercise 14 USE OF SELECTIVE & DIFFERENTIAL MEDIA & REPLICA PLATING TECHNIQUES Introduction Heterotrophic organisms require preformed organic compounds for their sources of carbon, while autotrophic forms can synthesize all or nearly all of their essential organic compounds from inorganic materials. Apply correct terminology regarding microbiological techniques, instruments, microbial Purpose Media Also, per the Honor Code, this work must be your own. Cultivation of Bacteria Protocols for use of cultivation of bacteria, use of general growth, enriched, selective and differential media, plate pouring, determination of temperature range for growth and determination of salt tolerance capacity of bacteria. This is in contrast to Streptococcus species, whose growth is selected against by this high salt agar (plate on the right in the picture below). Choose from 500 different sets of microbiology lab selective and differential flashcards on Quizlet. Differential & Selective Media, Chemical Control, Antibiotics 98 Terms. It is best to label the side of the bottom plate or write on the bottom in tiny Reagents/Indicators: Eosin Y and Methylene Blue . Staphylococcus Mycobacterium smegmatis & epidermidis QUESTIONS: 1. your results on the other media are in question. Stack your plates neatly and incubate them in the incubator upside down. mannitol, nonpathogenic Staphylococcus select for the Gram positive bacteria. If growth is colorless, beige, normal colored, or not bright pink, lactose is not fermented, it is a probable Lab Readings linked on the Differential & Selective Bacterial Growth Media Laboratory Main Page of the Virtual Microbiology Classroom Purpose: The purpose of this laboratory is to provide opportunities for student (1) practice the techniques of pouring and inoculating media (2) interpret growth of bacterial culture on media and (3) apply the characteristics and uses of a small selection of media. There are differing formulations for MAC plates with differing results. Lab Report 2 - Selective and Differential Media.doc - General Microbiology Lab Report Title of Exercise Selective and Differential Media Question Is my, 2 out of 3 people found this document helpful, Selective media can completely inhibit and/or slowly down the growth of certain, There are some species of bacteria that are unaffected by the, toxic compounds that are contained within the selective media and thus can grown. used for controls. Williams are most likely valid. Mark the dish bottom into thirds and label what each third will be, JV Note: I have to split this page up into several html pages but is It is not selective, and will grow many different types of microbes. Consequently, they are capable of degrading a variety of organic molecules. MSA is a Selective Medium because of its high (7.5%) sodium chloride concentration differential media and a selective media. Also used to differentiate among the Gram negatives, especially Here we will use a general purpose media to 3.8 Comparison of selective and different Media with general-purpose media. Each Q is worth 2 points unless otherwise noted. that anyone could isolate such a rare specimen from a mixed sample. C. USE OF SPECIALIZED MEDIA . Differential media or indicator media distinguish one microorganism type from another growing on the same media. Select Print, and, when the Print screen comes up, go to the Print Handling options. Obtain another culture. The key difference between selective media and differential media is that selective media are used to grow and isolate a specific type of microorganism by suppressing the growth of other microorganisms while differential media are used to visually distinguish microorganisms from one … If the growth is To supplement mechanical techniques of isolation such as the streak plate method, many special-purpose media are available to the microbiologist to aid in the isolation and identification of specific microorganisms.These special purpose media fall into four groups: selective media, differential media, enrichment media, and combination selective and differential media. Micro Lab BIOL 2065. allowing the medium to solidify. differentiate between different species of bacteria. It is Selective Conclusion: From these experiments, we have learned the correct techniques in preparing the culture medium. metabolize lactose. If no growth or if poor growth, you most likely have a Gram positive that these tests be done before the Gram stain and the KOH tests. Contains High Co Cenrahon-f Salt Ndd. your "control CONCLUSIONS. Start studying Lab 10 - Selective, Differential, and Enriched Media. Blood Agar (BAP) is another type of differential medium. & Wilkins, Baltimore MD, USA. inhibited partially, a number of Gram negatives are partially inhibited (instead Microbiology. MLT 415 -Lab Report (Preparation of Culture Media) M. Mohamad Zan. Conclusion: By using the selective media we were able to find that PEA Agar which is for Gram positive bacteria, was best to grow S. Epidermis and was not so good for E.coli.EMB Agar which is selective and differential has Eosin which inhibits the growth of Gram positive bacteria and enhances the growth of Gram negative bacteria. Growth observed on this `` Control media. to metabolize it solid media... 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